Stacked GHB614 x LL25 cotton was produced by crossing genetically engineered cotton lines to make the stacked event resistant to glyphosate (GHB614) and glufosinate (LL25). Owing to further crossings with MON15985 cotton, the final stacked plants produce two insecticidal proteins (Cry1Ac and Cry2Ab2). In addition, the plants produce proteins that confer resistance to antibiotics (NPTII and AAD) as well as the GUS protein that was used as a histochemical marker during product development.